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KMID : 0357319950300050563
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Journal of the Korean Society for Microbiology
1995 Volume.30 No. 5 p.563 ~ p.572
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The Quantitation of Myelin Basic Protein using Elisa in The Mouse Brain Derived Vaccine: Hantavax TM and Japanese Encephalitis Vaccine
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김 훈
장양석/안창남/김희철/이호왕
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Abstract
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The vaccine's neural tissue substrate has raised concern about the possibility of postvaccination neurological side effects. Myelin basic protein is one of the major central nervous system proteins and can induce many of symptoms of experimental
allergic encephalomyelitis. In the mouse brain derived vaccines such as Hemorrhagic fever with renal syndrome(HFRS) vaccine & Japanes encephalitis vaccine, myelin basic protein content is controlled below 2ng per ml; the amount of myelin basic
protein
considered to have an encephalitogenic effect in a guinea pig test system. To measure the quantity of myelin basic protein in the mouse brain derived vaccines, that HantavaxTM prepared from suckling mouse brain and Japanese encephalitis vaccine
prepared
from weanling mouse brain, an enzyme immunoassay using a double-antibody solid phase technique for myelin basic protein has been developed. In order to test the homogeneity of standard mouse myelin basic protein prepared from ICR adult mouse
brain,
urea
SDS PAGE, western blotting and high performance liquid chromatography were performed. The purity of mouse myelin basic protein was about 80%. In the Japanese encephalitis vaccines, content of myelin basic protein was 1.22-0.84ng per 1ml(1 dose)
and
HantavaxTM was below 0.05ng per 0.5ml(1 dose) calculated by ELISA.
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